The discussion focuses on determining the value of epsilon (ε) in Beer's Law, represented by the equation A = εbc, where A is absorbance, b is the path length, and c is the concentration. The user seeks clarification on how to extract epsilon from a linear graph plotting absorbance against concentration. Key insights include recognizing that the slope of the line in the graph corresponds to ε multiplied by the path length (L), and understanding that the y-intercept (b) represents absorbance at zero concentration.
PREREQUISITESChemistry students, laboratory technicians, and educators seeking to understand the application of Beer's Law in quantitative analysis of solutions.
Hikari said:Absorbance vs. Concentration
Gee. Let me think. What do those symbols and letters mean/stand for?Hikari said:A = Epsilon x L x c