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Best vectors for gene mapping (FISH)?

  1. Mar 10, 2015 #1
    If you were to have clones of genes you wished to use for FISH in the form of a plasmid, cosmid, BAC and YAC, which would be best for gene mapping? I'm unsure as to what the distinction would be between these types particularly for use in FISH. Which is most commonly used and why?

    Thanks
     
  2. jcsd
  3. Mar 13, 2015 #2
    I will try to help.

    Plasmids are from bacteria source,cosmid from viruse,YAC from yeast. So it depends from where and which gene are you want to see with FISH? Difference is expression promoters..ect,then there is an issue of scale or number of genes in those systems,YAC is biggest, so bacteria is procaryotic and yeast eucaryotic it is big difference as you probably know.
     
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