Can DNA repair enzymes reverse oxidative damage and combat aging?

[bioquest]

When you said overexpressing every repair mechanism did you mean only the ones that belonged to the species/type of animal they belonged to- you weren't saying you should add repair mechanisms from other species/animals were you? If I was adding proteins or something via endosomes to increase those repair mechanisms what else would I have to add other than proteins ie what repair things were you referring to other than the proteins? I want to do all the reading I'm just trying to understand a bit better about what you were saying about that relating to that specifically

also did you say overexpress every repair mechanism, to deal with just the oxidative types of dna damage, or to deal with all/some more types of dna damage? (if so what types) ty

 

[Mike H]

I meant all of the naturally occurring DNA repair mechanisms to that organism, not foreign ones. That is all. I had no thought of adding anything to the organism via liposomes or injections or any such means. I simply meant that you would need to upregulate/overexpress every naturally occurring DNA repair mechanism native to that organism.

There are other types of DNA damage than just oxidative types. They are described in the vast literature on DNA replication and repair with a great deal of care and knowledge.

 

[bioquest]

Were you only talking about overexpressing the known, not unknown proteins/mechanisms that do dna repair though?

if I already asked that sorry, I'll go through the thread again, my computer is just loading really slowly right now so I didn't go through the thread to see if I already asked that

 

[Mike H]

How can someone discuss overexpressing unknown proteins or initiating a process through unknown mechanisms by definition, as they're unknown? It doesn't make sense.

Anyway, now I'm really done. This thread has gone on for three weeks, and the amount of material which we've cited or have pointed you in the direction of will last you months, if not longer, given the citations/references at the various websites and papers. I wish you luck with your work, though.

 

[bioquest]

yah sorry I was being OCD and I need to stop being ocd

 

[bioquest]

so does this mean, that the proteins that would repair mismatches and the proteins that would repair oxidative dna damage do not cause exactly the same thing when they repair the dna? You said they may have a similar general strategy for the fixing their individual problem quote from earlier in the thread:

"there may be some overlap in the proteins used in the cleanup stage (there are a couple of different DNA polymerases, I can never keep them straight in my mind), but the substantive work of identifying and snipping out the offending nucleotide(s) are being done by very different sets of proteins. They may have a similar general strategy for fixing their individual problem, but they identify different problems in different ways. I don't remember all of the details of the biochemistry involved, that's something you would be better served by going to a textbook as they usually have the chemical structures all sketched out very neatly."

Would other proteins, not just the ones that do the actual repair (such as signalling proteins etc) have to be overexpressed too? we're talking about overexpressing all known dna repair proteins, not just the ones that solve oxidative damage but the ones that solve mismatched bases etc too right?

 

[bioquest]

Is it possible to transfer hundreds of proteins/tons of proteins (how many?) at a time via endosomes, nanoparticles, viruses, combination of them etc? (Ie before the first proteins of that much disappeared) I know it would be temporary...but also what would be the most efficient delivery methods/combinations of them and why? thanks (to deliver base/nucleotide excision repair proteins + possibly others, specifically)

 

[bioquest]

my friend messed me up about mismatch repair but um..what i was wondering is can the issue of DNA damage contributing to aging be solved by replacing the genes that control etc dna repair with undamaged genes? Would that be more efficient (to solve the problem etc of dna damage contributing to aging) than just increasing the amount of ubiquitin proteins/proteins that clean up degraded proteins? Even if you could sequence a cell; there would be too much dna damage that contributes to aging in the cell to fix it all with gene therapy right?