Change in internal energy => change in rest mass

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AcidRainLiTE
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Consider the following situation. Two particles are initially separated by some distance and are then pulled further apart, thus increasing the potential energy of the system (due to the gravitational force of attraction between them). If I am correct in understanding special relativity, then the system's rest mass was initially less than that of the final system.

So this should mean that it would be harder to accelerate the system of particles after they have been pulled farther apart than it was to accelerate them initially. In essence, you have to push on the particles harder to get them up to some given speed. In what ways has this been experimentally tested? Can anyone provide references to particular experiments or papers in which the experiments were described?

I am quite intrigued by the fact that separating particles would somehow make them more resistant to changes in motion.

Thanks for your information.
 
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It's a fairly everyday observation.
The gravitational force is too weak to show any easily measurable effect. But the nuclear force much stronger and shows the effect in virtually every nuclear interaction.

Simply compare the mass of an alpha particle (Helium nucleus) with that of it's component parts. (two protons, two neutrons) It weighs about 4.00150 amu instead of 4