Cis and Trans Acting Elements/Sites

  • Thread starter Thread starter rockind78
  • Start date Start date
Click For Summary
SUMMARY

Cis and trans acting elements are crucial components in genetic regulation, with cis acting elements located on the same DNA molecule and trans acting elements found on different molecules. A prime example is the lac operon, where LacI and lacO serve as cis acting elements. In a wild type strain, knocking out lacI and complementing it with a plasmid carrying lacI demonstrates the functionality of trans acting elements, as the lacI on the plasmid operates independently of the original DNA molecule.

PREREQUISITES
  • Understanding of genetic regulation mechanisms
  • Familiarity with the lac operon model
  • Knowledge of plasmid functionality in genetic complementation
  • Basic concepts of molecular biology terminology
NEXT STEPS
  • Research the role of cis acting elements in gene expression regulation
  • Study trans acting factors and their mechanisms in genetic control
  • Explore the implications of lac operon mutations on bacterial metabolism
  • Investigate other examples of cis and trans acting elements in different organisms
USEFUL FOR

Geneticists, molecular biologists, and students studying gene regulation mechanisms will benefit from this discussion on cis and trans acting elements.

rockind78
Messages
78
Reaction score
0
I am a bit confused I guess about exactly what these are. If anyone could offer up an example of each, I would be very grateful. I did find this much:

http://cancerweb.ncl.ac.uk/cgi-bin/omd?query=cis+acting
 
Last edited by a moderator:
Biology news on Phys.org
Monique ask the same question in this thread
https://www.physicsforums.com/showthread.php?s=&threadid=9011&highlight=cis+trans

Basically, cis is an element that is on the same DNA molecules and trans is something on a different molecules.

Best example is with the lac operon. In wild type strain, LacI and lacO are cis acting elements. If you knock-out lacI and complement the defective strain with a plasmid carrying lacI. The lacI on the plasmid is an example of a trans acting element.
 
Last edited:

Thread 'What causes the asymmetry in a symmetrically developing organism?'

As child, before I got my first X-ray, I used to fantasize that I might have a mirror image anatomy - my heart on the right, my appendix on the right. Why not? (Caveat: I'm not talking about sci-fi molecular-level mirroring. We're not talking starvation because I couldn't process certain proteins, etc.) I'm simpy tlakng about, when a normal zygote divides, it technically has two options which way to form. Oen would expcet a 50:50 split. But we all have our heart on the left and our...
View full post »

Similar threads

Graduate F.E.M and Hamilton's Principle (converting differential equations into integral equations)
  • · Replies 1 ·
Replies
1
Views
1K
I need help understanding this quote about recent Natural Selection
  • · Replies 20 ·
Replies
20
Views
3K
MHB The cofactors of elements for every determinant
  • · Replies 6 ·
Replies
6
Views
2K
Looking for a good source for problem sets
  • · Replies 1 ·
Replies
1
Views
937
Why Don't Insulin Needles "Damage" You or Hurt When Poking Yourself?
  • · Replies 37 ·
2
Replies
37
Views
5K
Globular cluster luminosity function
  • · Replies 3 ·
Replies
3
Views
2K
Graduate Calculating nonequilibrium Green's Functions
  • · Replies 1 ·
Replies
1
Views
1K
Is my understanding of the Nyquist theorem correct?
  • · Replies 4 ·
Replies
4
Views
2K
Computing the standard deviation in y
  • · Replies 4 ·
Replies
4
Views
2K
A mathematical description of the physics behind Aurora?
  • · Replies 3 ·
Replies
3
Views
2K