Discussion Overview
The discussion revolves around the preparation of protein solutions, specifically focusing on the ingredients and methods for creating solutions containing tributylphosphine, SDS, and urea. Participants explore the necessary concentrations and solvents for these solutions.
Discussion Character
- Technical explanation
- Conceptual clarification
- Debate/contested
Main Points Raised
- One participant requests recipes for making protein prep solutions, specifically mentioning 1 mM tributylphosphine, 1% SDS, and 10 M urea.
- Another participant explains the calculation of weights needed for 1 mM and 10 M solutions, noting that urea is solid while tributylphosphine is typically liquid.
- A participant inquires about the appropriate solvent for dissolving SDS, questioning whether to use sterile water or buffers.
- It is suggested that SDS and urea can be diluted in distilled deionized water, with a note that buffer requirements should be specified in the solution's name.
- One participant mentions that tributylphosphine is stored in N-methyl-2-pyrrolidine according to a reference from Sigma.
- Another participant advises checking the spec sheet for solubility information and contacting the manufacturer if the spec sheet is unavailable.
- A later reply emphasizes the use of nanopure water for protein chemistry and reiterates that SDS concentration is typically expressed as w/v.
Areas of Agreement / Disagreement
Participants express varying opinions on the appropriate solvents for the solutions and the specifics of preparing them, indicating that multiple views remain on these topics.
Contextual Notes
Some assumptions about the solubility of the chemicals and the definitions of concentration types (w/v) are not fully resolved, and there are references to specific products that may not be universally applicable.