Dilution Help: Finding the volume of water needed

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SUMMARY

The discussion focuses on preparing a 60 mL solution of 0.1 mol Albumin from a 2.5 mol stock solution using the dilution formula C1 X V1 = C2 X V2. The calculated volume of stock solution required is 2.4 mL. However, determining the volume of water needed for dilution is complex due to the non-additive nature of liquid volumes. It is emphasized that precise calculations require knowledge of the densities of the solutions involved, and standard laboratory practice dictates measuring the final volume of the diluted solution rather than relying on pre-calculated volumes.

PREREQUISITES
  • Understanding of the dilution formula (C1 X V1 = C2 X V2)
  • Basic knowledge of molarity and solution preparation
  • Familiarity with the concept of volume additivity in liquid solutions
  • Awareness of the importance of solution densities in calculations
NEXT STEPS
  • Research the concept of molarity and how to calculate it for different solutions
  • Learn about the properties of solutions, including volume additivity and density
  • Study laboratory best practices for preparing diluted solutions
  • Explore methods for accurately measuring solution densities
USEFUL FOR

Chemistry students, laboratory technicians, and anyone involved in solution preparation and dilution processes will benefit from this discussion.

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The objective: Acquire 60 mL of 0.1 mol Albumin. The only Albumin solution available is a 2.5 mol stock solution.

1)How many milliliters of the stock solution will you need to make the desired amount of the 0.1 M solution?
I used the
Code:
C1 X V1 = C2 X V2
formula to calculate an answer of 2.4 mL[/color]

2)How many milliliters of water will be needed?
This is where I'm stuck, I am aware I need to find the mass percent of water or something, but this is the only information given to me and my instructor has not given any examples.

If anyone could point me in the right direction it would be greatly appreciated.
 
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Simplest thinking yields V1 + V2 = 60 mL.

This is not 100% true, as volumes are not additive and final volume can be different from sum of volumes mixed, but as a first approximation, especially when solutions are not too concentrated, it works. So you can either use simplified approach using equation above, or answer that there is not enough information to precisely solve the question (you would need densities of all three solutions - water, 2.5 mol stock, 0.2 mol).

This is the reason why in lab you will never mix two precalculated volumes, standard procedure is to measure volume of the diluted solution and fill it up to desired final volume.

--
 
Thanks for the detailed advice, it was a great help!
 

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