Final Concentration: 40 µMol from .01mL Stock Solution

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SUMMARY

The discussion centers on calculating the final concentration of a solution after diluting a 40 µM stock solution. By adding 0.01 mL of the stock solution to 0.98 mL of water, the final volume becomes 0.99 mL. Using the dilution equation M1V1 = M2V2, where M1 is the initial concentration and V1 is the initial volume, participants confirm that the final concentration can be determined without changing the number of moles of solute.

PREREQUISITES
  • Understanding of molarity and concentration calculations
  • Familiarity with dilution equations, specifically M1V1 = M2V2
  • Basic knowledge of solution preparation techniques
  • Ability to perform unit conversions and volume calculations
NEXT STEPS
  • Study the principles of molarity and how to calculate it
  • Learn about the concept of dilution and its applications in laboratory settings
  • Explore examples of using the M1V1 = M2V2 equation in various scenarios
  • Investigate common laboratory techniques for preparing diluted solutions
USEFUL FOR

Students in chemistry, laboratory technicians, and anyone involved in solution preparation and concentration calculations will benefit from this discussion.

NCollege4Life
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if i have a 40 micromolar concentration of stock solution and add .01 mL of that solution to .98mL of water, what is my final concentration.

Thanks for any help!
 
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NCollege4Life said:
if i have a 40 micromolar concentration of stock solution and add .01 mL of that solution to .98mL of water, what is my final concentration.

Thanks for any help!

What is the final volume of your solution?
 
sorry! .99 mL
 
Does the number of moles in the final solution differ from the number of moles in initial solution?

--
methods
 
no, same
 
Last edited:
so any help?
 
Use the equation relating the initial molarity to the final molarity and volume:

M1V1 = M2V2
 
NCollege4Life said:
so any help?

My question was a hint. If amount of substance have not changed, why don't you calculate initial amount of substance and use concentration definition to calculate final concentration?

--
methods
 

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