Find the resolving power of a microscope in terms of its diameter

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SUMMARY

The discussion focuses on determining the resolving power of a microscope lens based on its diameter. Participants emphasize the conflict between achieving a short focal length for high magnification and a large diameter for maximum resolution. The lens maker's equation, specifically the formula 1/f = (n-1)(1/R1 + 1/R2), is critical for calculating the focal length, where n is the index of refraction (1.54 for glass). The final resolution is expressed in terms of the wavelength λ of light, with results indicating ranges of 0.8-1.3D for focal length and 1.0-1.6λ for the distance between resolvable points.

PREREQUISITES
  • Understanding of the lens maker's equation
  • Knowledge of optical sign conventions for lenses
  • Familiarity with Rayleigh's criterion for resolution
  • Basic principles of light and optics, including wavelength
NEXT STEPS
  • Study the lens maker's equation in detail, focusing on its application to converging lenses
  • Research optical sign conventions to avoid common pitfalls in calculations
  • Learn about Rayleigh's criterion and its implications for microscope resolution
  • Explore the relationship between lens diameter and focal length in optical systems
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Students in optics, physics enthusiasts, and professionals involved in microscopy and optical engineering will benefit from this discussion.

mew1033
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Find the resolving power of a microscope lens in terms of its diameter

Homework Statement


In this problem, we will find the ultimate resolving power of a microscope. First of all, in order to obtain a large magnification, we want an objective lens with a very short focal length. Second, in order to obtain maximum resolution, we also want that lens to have as large a diameter as possible. These two requirements are conflicting, since a lens with a short focal length must have a small diameter. It is not practical for a lens to have a diameter much larger than the radius of curvature of its surfaces. Otherwise, the lens starts looking like a sphere. So, let us assume that the objective lens has a diameter D equal to the radius of curvature of the two surfaces, like the lens in the figure.
(a) If the lens is made of glass with index of refraction 1.54, find the focal length f in terms of the diameter D of the lens.
(b) The distance between the sample to be observed and the objective lens is approximately equal to the focal length f . Find the distance between two points on the sample which can be barely resolved by the lens. Use the result from part (a) to eliminate f from the expression. You should find that D is
also eliminated from the expression and that the answer is given entirely in terms of the wavelength λ of the light. You may use the small angle approximation, sinθ ≈ tanθ ≈ θ.

The answer for part a is given as being in the range 0.8-1.3 ##D##
The answer for part b is given as being in the range 1.0-1.6##\lambda##

Homework Equations


I think that we will use the lens makers equation: ##1/f=(n-1)(1/R_1-1/R_2)## for part a. Then for part b, I think it's Rayleigh's criterion: ##sin\theta=\lambda/a##

The Attempt at a Solution



I'm completely stuck at part a... I'm not really sure what I should use for ##R_1## and ##R_2##.
I think if I got part a, part b would make more sense.


Thanks
 

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mew1033 said:

Homework Statement


I'm completely stuck at part a... I'm not really sure what I should use for ##R_1## and ##R_2##.
I think if I got part a, part b would make more sense.


Thanks

"So, let us assume that the objective lens has a diameter D equal to the radius of curvature of the two surfaces."
 
Do you mean set D equal to R1 and R2? If I do that, then (1/R1-1/R2) comes out to be 0. Then the focal length is basically infinity.
 
What is the sign convetion for ## R_1 ## and ## R_2 ## in the equation you are using?
 
I'm not sure what you mean by that, sorry...

I talked to another student and they said that when you use the lens-makers equation, if it's a converging lens then you do ##1/f=(n-1)(1/R_1+1/R_2)##

Looks like that was my problem.
 
mew1033 said:
I'm not sure what you mean by that, sorry...

I talked to another student and they said that when you use the lens-makers equation, if it's a converging lens then you do ##1/f=(n-1)(1/R_1+1/R_2)##

Looks like that was my problem.

That's the formula I would use. If it's convex to the outside it's +. So both faces are convex to the outside and so it's 1/R1 + 1/R2. It obviously makes sense too. A double-convex lens is obviously not equivalent to a flat piece of glass!

Some of these optial sign conventions seem bizarre but to be candid if you don't follow them sooner or later you get into trouble. Especially with object/image/virtual/real blah blah problems.
 
The book claims the answer is that all the magnitudes are the same because "the gravitational force on the penguin is the same". I'm having trouble understanding this. I thought the buoyant force was equal to the weight of the fluid displaced. Weight depends on mass which depends on density. Therefore, due to the differing densities the buoyant force will be different in each case? Is this incorrect?

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