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Fluorescence diffusion physics problem
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[QUOTE="cwraabe, post: 4686560, member: 505119"] [h2]Homework Statement [/h2] As a simple model, suppose you arrange for the intense bleaching pulse of light to illuminate a cell in a pattern of parallel planes. That is, immediately after bleaching (at time t=0), the remaining fluorescence intensity Θ(x) has the form Θ(x)= C(i) + C(1)sin(2pix/L) where the two C terms are constants with C(0) greater than C(1). The constant L is the periodicity of the bleach pattern. A. Show that the mean fluorescence intensity immediately after bleaching equals C(0). B. At later times, the fluorescence intensity has the form Θ(x)= C(0) + Δ(t)sin(2pix/L). Derive an expression for the diffusion constant D of the fluorescent molecules in terms of the measured quantity Δ(t). Assume that the region of interest is sufficiently small that you can approximate the cell as having infinite volume. [h2]Homework Equations[/h2] 1D diffusion equation perhaps? [h2]The Attempt at a Solution[/h2] obeys the 1D diffusion equation as the instructor told us this. We only need to calculate within 1 period, from 0 to L. [/QUOTE]
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Fluorescence diffusion physics problem
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