How Do You Calculate the Percentage Transmission Using the Beer-Lambert Law?

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SUMMARY

The discussion focuses on calculating percentage transmission using the Beer-Lambert Law, specifically for an aqueous solution of yeast nucleic acid with a concentration of 2.0 mol m-3 and an absorptivity of 25 m2 mol-1. To find the percentage transmission, one must first calculate the absorption per length by multiplying the concentration by the absorptivity. This value can then be applied in the Beer-Lambert equation to determine the percentage transmission at the specified wavelength.

PREREQUISITES
  • Understanding of Beer-Lambert Law
  • Knowledge of absorptivity and its units (m2 mol-1)
  • Familiarity with concentration units (mol m-3)
  • Basic algebra for calculations
NEXT STEPS
  • Study the Beer-Lambert Law in detail
  • Learn how to calculate absorption using concentration and absorptivity
  • Explore examples of percentage transmission calculations
  • Investigate the impact of wavelength on absorptivity
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Chemistry students, laboratory technicians, and anyone involved in spectrophotometry or analyzing solutions using the Beer-Lambert Law.

lagotuna
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An aqueous solution of yeast nucleic acid of length 2.0*10^-2m and concerntrated 2.0mol m-3 has an absorptivity of 25m2 mol-1 at a certain wavelength. calculate the percentages tranmission at this wavelength.

this is an example that i don't understant, please help me to simplify it and explain it more for me. you can send me as many example as possible.

thank you for your kind attention.
 
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You can multiply concentration and absorptivity to get the absorption per length. This can be used in the Beer-Lambert law.
 

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