To calculate a perpendicular line from two given points, first determine the slope of the line connecting those points using the formula (y2-y1)/(x2-x1). The slope of the perpendicular line is the negative reciprocal of this slope. While you can find the equation of the original line in slope-intercept form (y = mx + b), there are infinitely many perpendicular lines unless a specific intersection point is defined. The y-intercept (b) for the perpendicular line can vary, depending on where you want it to intersect the original line. Understanding these relationships allows for the calculation of various perpendicular lines based on the initial coordinates.