How to insert genes in a plant?

  • Thread starter Thread starter Richard_Steele
  • Start date Start date
  • Tags Tags
    Genes Plant
Click For Summary
SUMMARY

The discussion focuses on the methods for inserting genes from one plant species into another, emphasizing that this process requires a solid understanding of plant biology and genetics. Participants recommend joining a working group or educational program to gain hands-on experience. Key techniques mentioned include the use of gene guns for direct injection of plasmid DNA and the application of bacterial vectors for gene integration. The conversation highlights the importance of having the right background and equipment to successfully conduct genetic modifications in plants.

PREREQUISITES
  • Understanding of plant biology and genetics
  • Familiarity with plasmid design and construction
  • Knowledge of gene transfer techniques, including gene guns and bacterial vectors
  • Access to laboratory equipment for genetic modification
NEXT STEPS
  • Research the use of gene guns in plant genetic engineering
  • Learn about bacterial vectors and their role in gene integration
  • Explore educational programs in plant biology and genetics
  • Find online communities or working groups focused on plant genetic modification
USEFUL FOR

Biologists, genetic engineers, and researchers interested in plant genetic modification techniques and those seeking to develop new plant varieties through genetic engineering.

Richard_Steele
Messages
53
Reaction score
3
How can I insert pieces of other plant genes into another different specie of plant.
I want to create new plants, so I would know the process.
 
Biology news on Phys.org
The easiest way: Study biology, look for a working group doing something similar and join them. Long before you have the required knowledge to do this, you'll know several methods to transfer genes.
Plants are not lego, you cannot just put a random gene of one plant into another and get something interesting new.
 
When I worked in an experimental lab, we used viral vectors with C. elegans.
 
mfb said:
The easiest way: Study biology, look for a working group doing something similar and join them. Long before you have the required knowledge to do this, you'll know several methods to transfer genes.
Plants are not lego, you cannot just put a random gene of one plant into another and get something interesting new.

You know any group on the net that are involved in such task?
I've asked to some people near of me and no one is involved in any similar project, so I've to check the internet to find it.
 
Pythagorean said:
When I worked in an experimental lab, we used viral vectors with C. elegans.

Similar thing here except we used bacteriophages as viral vectors with bacteria.

I've never worked with plants before but a quick glance at Wikipedia tells me that they use gene guns that directly inject the genetic material through the cell wall in the form of metal particles coated with plasmid DNA, as well as a completely different process involving a species of bacterium that is able to integrate its plasmid DNA into a plant host.
 
You need a gene gun to insert plasmids into plant material, IIRC you have to prepare the seeds to be more receptive (this involves removing any outer shells) before firing plasmid loaded particles into them.

What background do you have in plant biology and genetics? Do you know how to design and construct plasmids? Do you have access to relevant equipment? If not then I advise that you seek out education and employment in this field as mfb has said.

Either way it would be helpful if you could provide information as to what your goal is and what means you have to do it so people can help you properly rather than having to guess whether to pitch their answers towards an amateur aiming to do work in their garage or a PhD student wanting to cross disciplines.
 
As child, before I got my first X-ray, I used to fantasize that I might have a mirror image anatomy - my heart on the right, my appendix on the right. Why not? (Caveat: I'm not talking about sci-fi molecular-level mirroring. We're not talking starvation because I couldn't process certain proteins, etc.) I'm simpy tlakng about, when a normal zygote divides, it technically has two options which way to form. Oen would expcet a 50:50 split. But we all have our heart on the left and our...

Similar threads

  • · Replies 7 ·
Replies
7
Views
2K
  • · Replies 2 ·
Replies
2
Views
2K
  • · Replies 3 ·
Replies
3
Views
2K
  • · Replies 6 ·
Replies
6
Views
3K
Replies
10
Views
5K
  • · Replies 7 ·
Replies
7
Views
7K
  • · Replies 3 ·
Replies
3
Views
2K
Replies
12
Views
5K
  • · Replies 2 ·
Replies
2
Views
2K
  • · Replies 1 ·
Replies
1
Views
3K