How to Prepare 0.1 M Tris-HCl Buffer: Step-by-Step Guide for Chem Gurus

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SUMMARY

This discussion focuses on preparing a 0.1 M Tris-HCl buffer and calculating the volume of 1 N HCl needed to adjust the pH to 8.0. The user has 80 mL of a 0.1 M Tris solution and seeks guidance on applying the Henderson-Hasselbalch equation for this purpose. The provided links to Chembuddy serve as resources for understanding pH calculations and buffer preparation.

PREREQUISITES
  • Understanding of buffer solutions and their components
  • Familiarity with the Henderson-Hasselbalch equation
  • Knowledge of molarity and normality concepts
  • Basic laboratory skills for preparing chemical solutions
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  • Study the Henderson-Hasselbalch equation in detail
  • Learn how to calculate pH adjustments in buffer solutions
  • Research the properties and uses of Tris-HCl buffers in biochemical applications
  • Explore online calculators for pH and buffer preparation
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Chemistry students, laboratory technicians, and researchers involved in buffer preparation and pH adjustment in biochemical experiments.

drunkenfool
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Would appreciate some assistance from some chem gurus :smile:

Trying to make the calculations necessary to prepare 0.1 M Tris-HCl buffer. I have 80mL of a 0.1 M tris solution and need to find the volume of 1 N HCl required to adjust the pH of Tris to 8.0. I believe I need to use the Henderson-Hasselbach equation but not sure how to set it up.

Thank you!
 
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