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Biology and Chemistry Homework Help
How to solve restriction mapping problems?
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[QUOTE="foldedelephants, post: 5621273, member: 608959"] [h2]Homework Statement [/h2] A piece of linear DNA 1000bp long is completely digested by four enzymes, E, B, P, and S. We are given the sizes of fragments (in bp) produced when each of the enzymes are used in isolation, and when they are used in different combinations: E : 227, 773 B : 150, 450, 400 P : 400, 600 S : 206, 794 E + B : 223, 227, 400, 150 E + P : 227, 373, 400 B + S : 56, 150, 344, 450 P + S : 194, 206, 600 [B][/B][h2]Homework Equations[/h2] Not applicable [h2]The Attempt at a Solution[/h2] I understand that with E + B, for example, as 227, 400, and 150 bp are present, enzyme B must have cut the 773 fragment into three pieces, 223, 400, and 150. I really don't know how to piece this information together to make a restriction map, it doesn't make any sense to me. [/QUOTE]
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How to solve restriction mapping problems?
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