Making new solution from stock solutions

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Discussion Overview

The discussion revolves around how to prepare a specific solution (75 mL of a Fixing Solution containing 40% ethanol and 10% acetic acid) from stock solutions of 100% acetic acid, 95% ethanol, and dH2O. The focus includes calculations for the required volumes of each component and considerations regarding the mixing process.

Discussion Character

  • Technical explanation
  • Mathematical reasoning
  • Debate/contested

Main Points Raised

  • One participant suggests using 31.6 mL of ethanol, 7.5 mL of acetic acid, and 36 mL of water to achieve the desired solution.
  • Another participant notes that volumes do not always add up when mixing solutions, recommending a method of mixing in a beaker and then adjusting the total volume in a graduated cylinder.
  • A third participant mentions that the calculated amount of ethanol may be incorrect, referencing a concentration calculator for clarification.
  • One participant provides a breakdown of the calculations for the amounts of each component based on the percentages, emphasizing that the total must add up to 100%.
  • Another participant adds that if the percentages are given as weight/weight (w/w), the calculations may not be straightforward due to non-additive volumes.

Areas of Agreement / Disagreement

Participants express differing views on the correct volumes needed for the solution and the method of mixing. There is no consensus on the exact amounts or the best approach to achieve the desired solution.

Contextual Notes

Participants highlight that the mixing of solutions can lead to volume discrepancies and that the calculations may vary depending on whether percentages are based on volume or weight. There is also mention of the rarity of 75 mL flasks, suggesting practical limitations in measurement tools.

rappa
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stock solutions: 100% acetic acid (liquid), 95% ethanol (liquid), dH20 (liquid)

1) 75 mL of Solution A: Fixing Solution (40% ethanol, 10% acetic acid)

they are saying if we have a 100% stock of acetic acid and 95% stock of ethanol, how do we make them into 40% ethanol and 10% acetic acid and combine to make 75mL

I was thinking maybe its: 31.6 mL of ethanol, 7.5 mL of acetic and 36 mL of water??
 
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Basically correct. Note that when mixing solutions, volumes do not always add up, so adding 31.6mL of ethanol, 7.5mL of acetic acid, and 36mL of water would not necessarily give you 75 mL. The best way would be to have some water in a beaker (~30mL maybe), add the ethanol, add the acetic acid, mix, then transfer to a graduated cylinder and add more water until the solution is 75mL.
 
Ethanol is a little bit off. See attachement - that's what CASC (concentration calculator) calculated.

Note: there are no 75 mL flasks (or at least they are very rare), so the idea of using measuring cylinder is better.

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75ml * 0.10 (10%) = 7.5ml
75ml * 0.40 (40%) = 30ml
75ml * 0.50 (50%) = 37.5ml

When they give it to you in percentages it's easy. Just make sure everything adds up to 100% after you calculate each individual amount needed.
 
ChmDudeCB said:
When they give it to you in percentages it's easy. Just make sure everything adds up to 100% after you calculate each individual amount needed.

If percentages are given as w/w it is not necesarilly that simple. Volumes are not additive.

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methods
 

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