Measuring the concentration of dye

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SUMMARY

This discussion focuses on measuring dye concentrations in water for the purpose of altering flower color. The participants emphasize the importance of using UV/Visible Spectroscopy to measure absorbance, which correlates to known dye concentrations. They highlight that dyes are highly concentrated compounds, necessitating precise measurement techniques to avoid significant errors in color perception. The conversation also touches on the challenges of measuring moles through evaporation and weighing, suggesting that absorbance measurement is a more practical approach.

PREREQUISITES
  • Understanding of UV/Visible Spectroscopy
  • Knowledge of absorbance and its correlation to concentration
  • Familiarity with molarity and mole calculations
  • Basic chemistry concepts related to solutions and solubility
NEXT STEPS
  • Research UV/Visible Spectroscopy techniques for dye concentration measurement
  • Learn about absorbance laws, specifically Beer-Lambert Law
  • Explore methods for preparing dye solutions and calculating molarity
  • Investigate the effects of dilution on color intensity and measurement accuracy
USEFUL FOR

Chemistry students, laboratory technicians, and researchers involved in dye chemistry or colorimetry who seek to accurately measure dye concentrations in solutions.

blahchick14
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I was wondering if someone could help me understand how to measure different concentrations of dye in water.
My experiment is to test out different concentrations of dye, that will be able to change the color of a flower.
I didnt want to use the simple way of putting 1 drop, 2 drop, etc...in water, and measuring it that way.
I think it can be measured in moles, but i only understand it when using sugar/salt, in water. I can't understand liquid in liquid, like dye in water.
can anyone help?
i'll appreciate it very much. :)
 
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Dyes are very very colored compounds, which means that only small amounts will be very apparent in solution. If you were going to measure moles, how would you do it? Would you evaporate the water off and weigh the solid (not easy) and do the math knowing the formula weight? Would you measure the absorbance of the solution and correlate it to known concentrations of the dye? (very easy) Most dyes are solids that are dissolved in solution. Concentrated solutions are often diluted in their final application since only a small error in measuring mass will cause a massive error in the apparent color.
 
UV/Visible Spectroscopy. Interest in absorbance.
 

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