Suppose that you are in a game with just one other player (the extension to several players is starightforward; the restriction is for clarity only) and have a set of strategies, from among you can choose one; the same is true for the other player.
Now, suppose that the other player chooses a strategy from his set; among your strategies there will be a subset that is the best response to the other player. This is not yet a Nash equilibrium, because the other player may change his strategy, to another one to which yours is no longer a best response but, if the other player's strategy is a best response to yours (this means that both strategies are best responses to each other), then you are at a Nash Equilibrium, in the sense that neither of you has any incentive to change strategies.
Consider the famous example of the Prisioner's Dilemma: if they both stay silent, they get the high reward; if they both confess, they both get a much lower reward and, if just one confesses, he gets the hightest reward, while the other gets nothing.
Where is the equilibrium? It's not the "stay silent" strategy, because both have an incentive to change. It's not either of the pairs (confess, stay silent), because the "stay silent" one has an incentive to change. It leaves the "confess": in this case, neither one has any incentive to change,