Optical Tweezers: Preparing Samples for RBC Trapping

Click For Summary
SUMMARY

This discussion focuses on the preparation methods for trapping red blood cells (RBCs) using optical tweezers. The user has created a sample with a mixture of 0.5 ml PBS, 0.5 ml distilled water, and a drop of blood but is experiencing issues with the RBCs not flipping during trapping. Recommendations include conducting a Google search for relevant articles, specifically referencing a study from Nature Communications that provides insights into optical tweezers and RBC trapping techniques.

PREREQUISITES
  • Understanding of optical tweezers technology
  • Knowledge of red blood cell (RBC) properties
  • Familiarity with sample preparation techniques in biophysics
  • Experience with PBS (Phosphate Buffered Saline) solutions
NEXT STEPS
  • Research the article "Optical Tweezers for Trapping Red Blood Cells" on Nature Communications
  • Explore advanced techniques for optimizing optical tweezers settings
  • Investigate sample dilution methods for RBC trapping
  • Learn about the effects of different buffer solutions on RBC behavior
USEFUL FOR

Researchers and students in biophysics, biomedical engineering, and anyone involved in optical trapping experiments, particularly those focusing on red blood cell manipulation.

henil
Messages
18
Reaction score
0
I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?
 
Biology news on Phys.org
henil said:
I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?
I did a Google search on optical tweezers for trapping red blood cells, and got lots of good hits. This was the first article on the list:

http://www.nature.com/ncomms/journal/v4/n4/full/ncomms2786.html

Maybe try doing that search, to see if you find what you need. :smile:
 
henil said:
I am currently working on project for trapping RBC's using optical tweezers but i think i am making a mistake in preparing the sample. Can anyone provide an appropriate sample preparing method ?

Does your trap work on microspheres? Do you have a dilute suspension of RBCs?
 
Andy Resnick said:
Does your trap work on microspheres? Do you have a dilute suspension of RBCs?
yes sir i do have worked on microspheres and i do have diluted blood with 1/2ml PBS, 1/2ml distilled DI water and a drop of blood. also the RBCs are not flipping .
i have tried changing the power supplied
 

Similar threads

  • · Replies 0 ·
Replies
0
Views
3K
  • · Replies 2 ·
Replies
2
Views
2K
  • · Replies 8 ·
Replies
8
Views
2K
Replies
8
Views
5K
  • · Replies 3 ·
Replies
3
Views
2K
  • · Replies 3 ·
Replies
3
Views
1K
  • · Replies 1 ·
Replies
1
Views
2K
  • · Replies 2 ·
Replies
2
Views
2K
Replies
12
Views
1K
  • · Replies 4 ·
Replies
4
Views
2K