Preparation of sodium phosphate buffer solution

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To prepare a 20 mM sodium phosphate buffer at pH 6.9 with 10 mM NaCl, the required amounts of sodium dihydrogen phosphate (NaH2PO4), disodium hydrogen phosphate (Na2HPO4), and sodium chloride (NaCl) for 1 liter of solution need to be calculated. The pH of the buffer can be adjusted using the Henderson-Hasselbalch equation, which relates pH to the ratio of the concentrations of the acidic and basic components of the buffer. The specific grams needed for each component can be determined using their molar masses: NaH2PO4 (approximately 119.98 g/mol), Na2HPO4 (approximately 141.96 g/mol), and NaCl (approximately 58.44 g/mol). The final concentrations should be verified to ensure the buffer solution meets the desired specifications for use at 37°C.
minimint89
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hi guys,

How can I prepare 20 mM sodium phosphate buffer (pH 6.9) containing 10 mM NaCl ? I mean how many grams of sodium dihydrogenphosphate, disodium hydrogenphosphate and sodium chloride are needed for 1 L of buffer solution. The buffer solution is prepared at room temperature (25C) and use at 37C.

Thank you :smile:
 
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