How does one prepare a 100ml of a 0.1M sodium phosphate buffer?

[g07l0989]

how would you prepare a 100ml of a 0.1M sodium phosphate buffer containing 0.5M NaCl,pH7.6 using henderson hasselbach equation??what chemicals would you use n how would you go about it

 

[mit]

Why do you use NaCl?

 

[g07l0989]

bu

cos i need the sodium ions anyway ignore the NaCL how would you do it??

 

[mit]

Buffersystems H3PO4/NaH2PO4 or NaH2PO4/Na2HPO4 or Na2HPO4/Na3PO4

 

[g07l0989]

any which would give a pH of 7.6 so i ges you would have to look at the pKa to decide which to use

 

[mit]

A handbook of analytical chemistry can help you with the mathematical explanation. For your pH range you can use the NaH2PO4/Na2HPO4 system range pH 6 - 8 with the middle value H+=Kz2 or -logKz2 =7.22 Greetings

 

[g07l0989]

thank you a lot that relly helped me thanks ciao...