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How does one prepare a 100ml of a 0.1M sodium phosphate buffer?

  1. Feb 27, 2008 #1
    how would you prepare a 100ml of a 0.1M sodium phosphate buffer containing 0.5M NaCl,pH7.6 using henderson hasselbach equation??what chemicals would you use n how would you go about it
     
    Last edited: Feb 27, 2008
  2. jcsd
  3. Feb 27, 2008 #2

    mit

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    Why do you use NaCl?
     
  4. Feb 27, 2008 #3
    bu

    cos i need the sodium ions anyway ignore the NaCL how would you do it??
     
  5. Feb 28, 2008 #4

    mit

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    Buffersystems H3PO4/NaH2PO4 or NaH2PO4/Na2HPO4 or Na2HPO4/Na3PO4
     
  6. Feb 28, 2008 #5
    any which would give a pH of 7.6 so i ges you would have to look at the pKa to decide which to use
     
    Last edited: Feb 28, 2008
  7. Feb 28, 2008 #6

    mit

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    A handbook of analytical chemistry can help you with the mathematical explanation. For your pH range you can use the NaH2PO4/Na2HPO4 system range pH 6 - 8 with the middle value H+=Kz2 or -logKz2 =7.22 Greetings
     
  8. Feb 28, 2008 #7
    thank you a lot that relly helped me thanks ciao....
     
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