Preparation of sodium phosphate buffer solution

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SUMMARY

To prepare a 20 mM sodium phosphate buffer solution at pH 6.9 containing 10 mM NaCl, you need to calculate the required amounts of sodium dihydrogen phosphate (NaH2PO4), disodium hydrogen phosphate (Na2HPO4), and sodium chloride (NaCl) for 1 liter of solution. The precise amounts are 2.4 grams of NaH2PO4, 2.4 grams of Na2HPO4, and 0.58 grams of NaCl. This preparation should be conducted at room temperature (25°C) and can be used at 37°C.

PREREQUISITES
  • Understanding of buffer solution chemistry
  • Knowledge of molarity calculations
  • Familiarity with pH measurement techniques
  • Basic laboratory skills for solution preparation
NEXT STEPS
  • Research the Henderson-Hasselbalch equation for buffer preparation
  • Learn about the properties and applications of sodium phosphate buffers
  • Explore methods for adjusting pH in buffer solutions
  • Investigate the effects of temperature on buffer capacity
USEFUL FOR

Chemists, biochemists, laboratory technicians, and anyone involved in preparing buffer solutions for biochemical experiments.

minimint89
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hi guys,

How can I prepare 20 mM sodium phosphate buffer (pH 6.9) containing 10 mM NaCl ? I mean how many grams of sodium dihydrogenphosphate, disodium hydrogenphosphate and sodium chloride are needed for 1 L of buffer solution. The buffer solution is prepared at room temperature (25C) and use at 37C.

Thank you :smile:
 
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