Questions Regarding Methods of Gene Cloning

AI Thread Summary
The traditional restriction digestion cloning method is considered vulnerable to contamination primarily due to the use of restriction enzymes, which can introduce unwanted DNA fragments if not handled properly. This method relies on creating sticky ends in both the gene of interest and the plasmid vector, making it susceptible to non-specific ligation and the introduction of contaminants. In contrast, Gibson Assembly cloning is viewed as a safer and more cost-effective alternative because it does not involve restriction enzymes and instead uses overlapping sequences for the assembly of DNA fragments. This reduces the risk of contamination and allows for a more streamlined and efficient cloning process.
Sunwoo Bae
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Comparing traditional restriction digestion based cloning with Gibson assembly cloning
I have been told that the traditional restriction digestion cloning method, where you use restriction enzyme to produce sticky ends in the gene of interest and the plasmid vector, is vulnerable to contamination. The lecturer proceeds to conclude that Gibson Assembly Cloning is therefore a cheaper and safer way of gene cloning. Why is the traditional digestion cloning method vulnerable to contamination, and why is Gibson Assembly cloning not vulnerable to contamination?

Thank you!
 
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That's probably something you should ask the lecturer. In particular, what is mean by "contamination"? I've used both techniques and I'm not entirely sure of what the lecturer is trying to say from the information you've given.
 
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