The discussion revolves around the search tools available for obtaining genomic DNA (gDNA), messenger RNA (mRNA), and complementary DNA (cDNA) sequences of specific genes, particularly focusing on the FOS gene in humans. Participants seek guidance on navigating databases and understanding the types of sequences available.
Participants express varying levels of understanding regarding the definitions and distinctions between mRNA, cDNA, and gDNA sequences. There is no consensus on the exact navigation steps within the NCBI database, and interpretations of terms like "translation" and "CDS" appear to differ.
Participants highlight the complexity of the NCBI database and the potential for confusion due to the terminology used. There are unresolved questions about the specific links to access different types of sequences and the definitions of certain terms.
This discussion may be useful for researchers, students, or anyone interested in bioinformatics, particularly those looking for guidance on accessing gene sequence data from online databases.
Anything that states mRNA in the definition (the sentence you see in the search results) will be a sequence from mRNA and the sequence deposit is either the mRNA or cDNA sequence. It should be noted under the feature option. To obtain either one all you have to do is find a software the complement the sequence.sobored said:1: Which link should i now click on to get the mRNA of the fos gene?
2. Which link should i now click on to get the cDNA of the fos gene?
Usually, anything that state complete or partial cds in the definition is genomic DNAsobored said:3. Which link should i now click on to get the gDNA of the fos gene?
sobored said:4. The sequence under "translation" is that the amino acid of a gene?
sobored said:5. What kind of sequence (mRNA, gDNA or cDNA) is under "CDS" of a gene?