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[sobored]

Search tools for ....

Where and how to get the gDNA, mRNA and cDNA sequences of a gene? Any search tools?



Thanks!

 

[iansmith]

NCBI genbank
http://www.ncbi.nlm.nih.gov/

 

[sobored]

Thanks for your answer!

I still have some questions regarding this search tool. Let us take for example the FOS gene for the human.
I came to this site:
http://www.ncbi.nlm.nih.gov/entrez/query.f...&list_uids=2353 [Broken]

I am now overwhelmed with all the infos and sequences in this site, besides there are alot of socalled mRNA and genomic sequences which i don't know which one of them is the mRNA, gDNA and cDNA for the FOS gene.

My questions:

1: Which link should i now click on to get the mRNA of the fos gene?

2. Which link should i now click on to get the cDNA of the fos gene?

3. Which link should i now click on to get the gDNA of the fos gene?

4. The sequence under "translation" is that the amino acid of a gene?

5. What kind of sequence (mRNA, gDNA or cDNA) is under "CDS" of a gene?


Hope for inputs!

Thanks!

 

[iansmith]

You link does not work so I did a search with "fos gene human"

1: Which link should i now click on to get the mRNA of the fos gene?
2. Which link should i now click on to get the cDNA of the fos gene?

Anything that states mRNA in the definition (the sentence you see in the search results) will be a sequence from mRNA and the sequence deposit is either the mRNA or cDNA sequence. It should be noted under the feature option. To obtain either one all you have to do is find a software the complement the sequence.
Example: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=6552332 [Broken]

3. Which link should i now click on to get the gDNA of the fos gene?

Usually, anything that state complete or partial cds in the definition is genomic DNA
Example: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=27802688 [Broken]
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=182734 [Broken]

4. The sequence under "translation" is that the amino acid of a gene?

Can you be more precise. I did not find "translation" in my search.

5. What kind of sequence (mRNA, gDNA or cDNA) is under "CDS" of a gene?

If you look at your search results, CDS usually means that the deposit sequence is genomic DNA data but if you look the deposit sequence CDS is the protein sequence.