Understanding Final Volume at Recovery in Transformation Efficiency Calculation

[Josh123]

I know that the formula to calculate the TE is

(#of transformants)/(g of DNA) * (Final volume at recovery) / (Volume plated)

but what exactly is "fianl volume at recovery"? Do I calculate the total # of pGal DNA, X-gal and recovery broth that I mixed up?

 

[Monique]

You need that to calculate what the fraction was that you plated. Did you plate all your transformation volume, or did you only plate part of it?