Way to force certain mutations to happen

[Virtuous]

I read that mutation is random, I am wondering if this is true, and if so; is it ever possible to by-pass it even if its completely inefficient method.

I guess I would be talking about DNA, I don't know much but anything.

Basically I am trying to see if there is a way to force certain mutations to happen, even if it takes several mutations to accomplish it.

 

[Moonbear]

I'm not entirely sure what you're trying to ask, but perhaps you're referring to something like transgenics or genetic engineering? It is still random, but you can enrich the media your cells are in with the gene you want to splice in, then use enzymes to open up the DNA, along with other manipulations like temperature changes, to increase the probability that the gene you want spliced in gets taken up by the cell.

 

[Biophreak]

Natural gene mutation really is a random event. Polymerases, which make new strands of DNA and RNA, are not perfectly accurate molecules. RNA polymerase has an error rate of approximately 1/10^3bp, and DNA polymerase has an error rate of approximately 1/10^6 bp. It is this minute error rate that allows natural selection to drive evolution.

As for your second question asking whether or not it is possible to cause a specific mutation (i think you mean phenotype) to appear, the answer would be, at the time, no. this is because directed mutation is not an available technique at the time. Using transposable elements, one can cause mutations to occur, but they are largely random, or confined to specific areas of the genome. However, this methodology is obsolete. We can cause specific phenotypes to show in an organism by directly adding or removing pieces of DNA- so there is no need to mutate a cell in order to cause a specific phenotype to appear.

The technique of inducing mutation is a commonly used protocol in reverse genetics- where one seeks to find the gene responsible for an attribute by mutating segments of DNA until the attribute is no longer observed.

Also, one can cause specific TYPES of mutations (U>A or C>G) to occur, because it is possible to predict what mutation a base pair will undergo if you subject it to UV light, mutagens, etc.

Hope this was helpful

 

[Virtuous]

Yeah that's perfect thanks.