What is the difference between non-reducing and reducing buffers?

[mountain]

what is the difference between a non-reducing and a reducing buffer?

and what does it mean with non-reducing and reducing?

thanks a bunch!

 

[Monique]

Are you familiar with redox reactions? Reduction and Oxidation. The cytoplasm of a cell is a reducing environment: an effect is that disulfide bonds within proteins get reduced and are thus hard to form.

DTT is a reducing agent and often added to reducing buffers.

 

[mountain]

thanks Monique! it seems like you know a lot of things are you a PhD?