What is the length of Chromatosomal DNA?

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SUMMARY

The length of chromatosomal DNA associated with nucleosomes has been a topic of debate, with key figures being 147 bp for the nucleosome core particle and varying estimates for the linker DNA protected by histone H1, ranging from 168 bp to 200 bp. Recent studies, including a notable structure published in Molecular Cell, suggest that approximately 200 bp is becoming the more widely accepted figure. However, the engagement of H1 with the nucleosome core particle remains controversial, and the scientific community has not reached a definitive consensus on this matter.

PREREQUISITES
  • Understanding of nucleosome structure and function
  • Familiarity with chromatin biology
  • Knowledge of histone proteins, specifically H1
  • Basic comprehension of molecular biology research methodologies
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  • Review the latest findings in chromatin structure from Molecular Cell
  • Examine the implications of linker histone H1 on nucleosome stability
  • Investigate the differences in nucleosome DNA length across various species
  • Explore ongoing research and emerging models regarding nucleosome interactions
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Researchers in molecular biology, biochemists studying chromatin dynamics, and anyone interested in the structural biology of nucleosomes will benefit from this discussion.

Tyto alba
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I see various sources:
1. Karp- Cell biology
2. some papers
3. Michael M. Cox - https://books.google.co.in/books?id=HmUdBQAAQBAJ&pg=PA338&lpg=PA338&dq=200+bp+or+168+nucleosome&source=bl&ots=TKH1Om-oJc&sig=r3q8RX96zLEtElX5r273jlCDJqY&hl=en&sa=X&ved=0ahUKEwjbm5HbyPLTAhVJuY8KHZkwAGkQ6AEIRzAI#v=onepage&q=200%20bp%20or%20168%20nucleosome&f=false
mention different values for the length of DNA associated with the nucleosomes (Octamer and H1); 168bp and 200bp. I'm a lost. Is there any paper that has settled this confusion once and for all?
 
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Canonically, the nucleosome core particle wraps 147 bp. Varying studies come up with different numbers protected by the linker histone (H1), for example, see paragraph 6 of http://www.nature.com/nsmb/journal/v5/n12/full/nsb1298_1025.html. A very recent structure of the ncp + linker histone used 197 bp of DNA, so ~200 bp may be the more generally accepted number now: http://www.cell.com/molecular-cell/fulltext/S1097-2765(17)30268-X I do know that there has been controversy over how H1 engages the ncp, and I'm not sure whether the field sees the Mol Cell paper as a definitive answer or if other groups have data in the works to support other structural models.http://www.cell.com/molecular-cell/fulltext/S1097-2765(17)30268-X
 
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