What makes the current coronavirus different from the others?

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Discussion Overview

The discussion centers around the unique characteristics of the current coronavirus (SARS-CoV-2) compared to previous coronaviruses and other viruses, particularly in terms of its transmission, mutation rates, and potential treatment options. Participants explore various scientific studies and theories related to the virus's behavior, shedding patterns, and implications for vaccine efficacy.

Discussion Character

  • Exploratory
  • Technical explanation
  • Debate/contested
  • Experimental/applied

Main Points Raised

  • Some participants note that SARS-CoV-2 appears to replicate more prolifically than the flu virus and possesses a proofreading enzyme that may limit its mutation rate.
  • Others discuss the ease of transmission of the virus, particularly due to high levels of viral shedding in asymptomatic individuals early in the infection.
  • A study suggests that SARS-CoV-2 may enter host cells through a novel route involving the CD147 protein, which differs from previous coronaviruses that primarily used the ACE2 receptor.
  • Concerns are raised about the implications of the virus's stability for vaccine development, with some participants questioning how this might affect long-term immunity.
  • Participants express uncertainty regarding the timeline and processes for clinical trials of existing drugs like Meplazumab for treating COVID-19, noting that while some phases may be expedited, efficacy still needs to be established.
  • There is speculation about the long-term efficacy of vaccines given the virus's potential for stability and mutation under certain conditions.

Areas of Agreement / Disagreement

Participants express a range of views on the characteristics of SARS-CoV-2, with no consensus on the implications of its mutation rate or the effectiveness of proposed treatments. The discussion remains unresolved regarding the long-term efficacy of vaccines and the specifics of clinical trial processes.

Contextual Notes

Some claims about the virus's behavior and treatment options are based on preliminary studies or non-peer-reviewed sources, indicating a need for further validation. The discussion also highlights the complexity of viral behavior and the challenges in understanding its implications for public health.

  • #91
What is a viral ferment?

At any rate, PCR uses a special DNA polymerase that is thermally cyclable in the amplification step. As the name suggests, DNA polymerase amplifies DNA, not RNA.
 
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  • #92
Also, in general RNA is fragile, while DNA can contain equivalent information but is chemically more stable.

Question: would RNA survive the heating step in PCR? When RNA has to be preserved it is common to keep samples cold, which is why I think many versions of the COVID-19 swab test require samples to be refrigerated, eg. https://stanfordhealthcare.org/heal...covid-19-test/covid-19-molecular-testing.html "Specimen must be kept at 2-8°C (refrigerated/cool) or frozen"

There seems to be an all RNA-version of PCR https://www.pnas.org/content/113/35/9786 (not sure how practical this is).
 
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  • #93
Stability and ability to sustain more heat make sense. Thanks
 

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