Exonic primers are short, single-stranded DNA sequences that are designed to specifically bind to and amplify a specific region of DNA that is located within an exon, which is a coding region of a gene.
Exonic primers are designed to target and amplify specific regions of DNA that are located within exons. This means that they can greatly enhance the efficiency and accuracy of gDNA amplification, as they specifically target and amplify the coding regions of a gene.
No, exonic primers are specifically designed to target and amplify coding regions of DNA, meaning they will not affect non-coding regions. This ensures that only the desired regions of DNA are amplified during the PCR process.
While exonic primers can greatly improve the efficiency and accuracy of gDNA amplification, they are not always necessary. In some cases, non-specific primers can be used to amplify a larger region of DNA that includes both coding and non-coding regions.
Choosing the right exonic primers depends on several factors, including the target gene, the desired region to be amplified, and the specific experimental conditions. It is important to carefully design and test primers to ensure they are specific and efficient for the desired amplification.