- #1
kubikat
- 19
- 0
I have a question concerning optical tweezers.
How do you make sure that the focal plane of the beam and the laser are the same..?
In other words, I can see the laser on my CCD image when it is reflected off the cover slide, but if I see the reflection of the laser then the beads that are stuck to the cover slide are not in focus... If the beads are in focus then I can not see the laser...
I have read papers on it and they all mention that issue and just say that it should be resolved... but they don't say how. I do realize that it is probably something trivial, but I just can not figure it out. Any hints will be greatly appreciated.
How do you make sure that the focal plane of the beam and the laser are the same..?
In other words, I can see the laser on my CCD image when it is reflected off the cover slide, but if I see the reflection of the laser then the beads that are stuck to the cover slide are not in focus... If the beads are in focus then I can not see the laser...
I have read papers on it and they all mention that issue and just say that it should be resolved... but they don't say how. I do realize that it is probably something trivial, but I just can not figure it out. Any hints will be greatly appreciated.