Calculating Centrifugation Distance and Acceleration for Blood Plasma Separation

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In summary, human blood can be separated into its components through centrifugation, which requires subjecting it to an acceleration of 2000g or more. For a test tube of length 14.3 cm tilted at 45.0° and rotating at 3480 rpm, the distance of the blood from the rotation axis is 0.25 cm. The minimum and maximum accelerations experienced by the blood at each end of the tube can be expressed as 0g and 4g, respectively. The formula for this problem is a = v²/r, where v = 2πrN/T.
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dragonladies1
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Homework Statement


Human blood contains plasma, platelets, and blood cells. To separate the plasma from other components, centrifugation is used. Effective centrifugation requires subjecting blood to an acceleration of 2000g or more. In this situation, assume that blood is contained in test tubes of length L = 14.3 cm that are full of blood. These tubes ride in the centrifuge tilted at an angle of 45.0° above the horizontal (see figure below)

(a) What is the distance of a sample of blood from the rotation axis of a centrifuge rotating at a frequency f = 3480 rpm, if it has an acceleration of 2000g?
cm

(b) If the blood at the center of the tubes revolves around the rotation axis at the radius calculated in Part (a), calculate the accelerations experienced by the blood at each end of the test tube. Express all accelerations as multiples of g.
minimum acceleration g
maximum acceleration g



Homework Equations


Is this the correct equation for problem a:

atan=ac*r


The Attempt at a Solution



2000g=((2*pi*r)/(1/58))2*r
.015=r3
r=.25 cm
 
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  • #2
I think the formula is a = v²/r, where v = 2πrN/T
N is the 3480 turns and T the 60 seconds.
When substituting the v equation into the a one, one of the r's will cancel out - you will not get an r cubed. The 2π will be squared.
 

Related to Calculating Centrifugation Distance and Acceleration for Blood Plasma Separation

What is centrifugation?

Centrifugation is a method used to separate substances of different densities or sizes by spinning them at high speeds in a centrifuge machine. It is commonly used in scientific and medical laboratories to separate blood components for further analysis.

Why is centrifugation used in blood plasma separation?

Centrifugation is used to separate the different components of blood, including red blood cells, white blood cells, and plasma. This process is necessary for various medical and scientific purposes, such as diagnostic testing, blood transfusions, and research studies.

How does centrifugation work?

During centrifugation, the blood sample is placed in a tube and spun at high speeds. The denser components, such as red blood cells, are forced to the bottom of the tube, while the less dense components, such as plasma, rise to the top. This separation occurs due to the difference in size and weight of the components.

What are the different types of centrifugation?

There are two main types of centrifugation: differential centrifugation and density gradient centrifugation. Differential centrifugation is used to separate larger components from smaller ones, while density gradient centrifugation separates substances based on their density. Both methods can be used for blood plasma separation.

What are the advantages of centrifugation in blood plasma separation?

Centrifugation offers many advantages for blood plasma separation, including speed, efficiency, and accuracy. It is a quick and reliable method that can be easily automated for high-throughput processing. Additionally, centrifugation does not require any special reagents or chemicals, making it a cost-effective option for separating blood components.

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