Excitation and Absorption spectra

[birdmani]

Hello,

am working with luminescence spectra, including excitation and emission spectra. When you makes an initial characterization of a sample should be done first?
an absorption spectrum?
At what wavelength excitation should be performed to see the desired effect?
Particularly when working with doped samples. for example in my case I work with Eu3 +. This emission is 580, 592, 614, 653, 702 nm, which should be the excitation wavelength longuitud?
Is there a reference book on how to perform the characterizations?
What other information can be obtained from this type of spectra?
Thanks for any info you can give me

 

[Valerie Park]

.When characterizing a sample, the best way to begin is usually to acquire an absorption spectrum. This will allow you to identify the different compounds present in the sample and their relative concentrations. Once this is done, you can then determine the emission spectra of the sample by performing excitation at the wavelengths corresponding to the absorption peaks. For your particular case with Eu3+, the excitation should be performed at the wavelengths corresponding to the emission peaks which are 580, 592, 614, 653, and 702 nm. Information that can be obtained from luminescence spectra includes the identity of the compounds present in the sample, their relative concentrations, and the energy level transitions (from excited to ground states) that occur when the sample is exposed to light. Additionally, luminescence spectra can also be used to determine the quantum yields of the sample and to quantify the amount of light energy absorbed and emitted by the sample. For more information on luminescence spectroscopy, there are many excellent reference books available, such as "Luminescence Spectroscopy" by Robert J. Huggins and "Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules" edited by Joseph R. Lakowicz.