- #1
ericvon11
- 10
- 0
Hi all,
I was given an unknown in a recent lab and determined it was lactose. However, I'm not quite sure why it reacted the way it did with the reagents used.
I used Red tetrazolium and NaOH which oxidizes aldoses and ketoses. Lactose is made of glucose and galactose, both of which are aldoses. I was wondering first off what NaOH would do in this procedure and if both are aldoses, why doesn't it react with Red tetrazolium? I only got a pink tint when I heated it. But since both are aldoses they should reduce the red tetrazolium right?
I was given an unknown in a recent lab and determined it was lactose. However, I'm not quite sure why it reacted the way it did with the reagents used.
I used Red tetrazolium and NaOH which oxidizes aldoses and ketoses. Lactose is made of glucose and galactose, both of which are aldoses. I was wondering first off what NaOH would do in this procedure and if both are aldoses, why doesn't it react with Red tetrazolium? I only got a pink tint when I heated it. But since both are aldoses they should reduce the red tetrazolium right?