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evidenso
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Raman spectroscopy of protein please help
Hey any Raman experts out there?
Im doing Raman spectroscopy of a protein called c-reactive protein (in aquous solution) using 532nm 10mW and the protein placed in a nmr tube. I can nicely get a spectrum of benzene and cyclohexane, but when I am doing Raman on the protein, the spectrum chanches dramatical for every measurement (flourescence like). how can this be? and how can i test if I have destroyed the protein?
MB
Hey any Raman experts out there?
Im doing Raman spectroscopy of a protein called c-reactive protein (in aquous solution) using 532nm 10mW and the protein placed in a nmr tube. I can nicely get a spectrum of benzene and cyclohexane, but when I am doing Raman on the protein, the spectrum chanches dramatical for every measurement (flourescence like). how can this be? and how can i test if I have destroyed the protein?
MB