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i am looking for a tool where i can designe degenerate PCR primers.
by the way i have read about degenerate PCR, but find it hard to understand, so please explain these for me;
in standard PCR mixture we have a forward and reverse primer for our target, but how many primers do i need in a degenerate PCR mixture? besides how does the degenerate primers work? when we induce wobble in the primer, how come one of the specific one is chosen among the wobbles? or how does it actually work to make our pcr product? *extremely confused*
hope for ideas!
thanks alot!
by the way i have read about degenerate PCR, but find it hard to understand, so please explain these for me;
in standard PCR mixture we have a forward and reverse primer for our target, but how many primers do i need in a degenerate PCR mixture? besides how does the degenerate primers work? when we induce wobble in the primer, how come one of the specific one is chosen among the wobbles? or how does it actually work to make our pcr product? *extremely confused*
hope for ideas!
thanks alot!
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