Does arsenate inhibit glyceraldehyde 3-phosphate dehydrogenase?

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Arsenate closely resembles phosphate, leading to its incorporation into glyceraldehyde-3-phosphate (G3P) by the enzyme GAPDH, which inhibits the enzyme's function. This results in the formation of an unstable arsenate ester that rapidly hydrolyzes in aqueous solutions, preventing the production of the intended product and causing the enzyme to consume G3P without yielding any output. For further details, the study by Crane and Lipmann from 1952 discusses the effects of arsenate on aerobic phosphorylation and provides additional references for deeper insights.
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I know it resembles a phosphate. Did a search it was very vague. Thanks :smile:
 
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Apparently arsenate "inhibits" GAPDH because arsenate is so similar to phosphate that the enzyme incorporates arsenate into G3P rather than phosphate. The arsenate ester that GAPDH creates, however, almost instantaneously hydrolyzes in aqueous solution, so the enzyme ends up consuming G3P without producing the product.

See Crane and Lipmann. 1952. The effect of arsenate on aerobic phosphorylation. J. Biol. Chem. 201: 235. http://www.jbc.org/content/201/1/235.full.pdf+html and the papers it references in the introduction.
 

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