Finding mass of glucose in standard solutions

In summary, the conversation is about an experiment using thin layer chromatography to separate sugars in a sample. The participants are given a glucose standard solution with a concentration of 1mg/ml and three standard sugar solutions (glucose, fructose, and maltose) with a concentration of 10mg/ml in 10% v/v isopropanol. They are tasked with finding the μg of glucose (reducing sugar) in each of the standards. There are no specific equations to be used, only conversions.
  • #1
DespicableMe
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Homework Statement



This is for an experiment that separates sugars in a sample using TLC.
We are given a glucose standard solution with a concentration of 1mg/ml as well as 3 standard sugar solutions (glucose, fructose and maltose) all with the same concentration (10mg/ml) in 10% v/v isopropanol)

We are asked to find μg of glucose (reducing sugar) in each of the standards.

Homework Equations


I don't think there are any equations, just conversions.

The Attempt at a Solution


Convert the mg/ml to μg/ml or to μg/μl
But I'm not sure if I'm overthinking it or if it's the way the question is worded because it's worth only 1 mark.
 
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  • #2
Hard to say anything not knowing how the question is worded. From your description it can be anything and everything.
 
  • #3
We're given a juice sample and we use thin layer chromatography to identify the sugars in there. They gave us the concentrations of the standard sugar solutions and we were asked to find the μg of glucose (the reducing sugar) in each of the standards. They said to refer to the glucose concentration given to us which was 1 mg/ml. The concentration of the standards were 10 mg/ml though.
 
  • #4
From what you wrote there is no glucose in the other standard solutions.
 
  • #5


As a scientist, the first step would be to understand the objective of the experiment and the materials provided. In this case, the objective is to separate sugars in a sample using TLC and the materials provided are a glucose standard solution and 3 other standard sugar solutions with the same concentration in isopropanol.

To find the mass of glucose in each standard solution, we can use the equation: mass = concentration x volume. In this case, the concentration is given in mg/ml, so we will need to convert it to μg/ml or μg/μl depending on the desired unit. This can be done by multiplying the given concentration by 1000 (since 1 mg = 1000 μg).

Once we have the concentration in the desired unit, we can then multiply it by the volume of the standard solution provided. This will give us the mass of glucose in each standard solution.

For example, to find the mass of glucose in the glucose standard solution (1mg/ml), we would first convert the concentration to μg/ml by multiplying it by 1000, giving us 1000 μg/ml. Then, if we are given a volume of 1 ml of the standard solution, we would multiply 1000 μg/ml by 1 ml to get a mass of 1000 μg of glucose in the glucose standard solution.

It is important to pay attention to the units and conversions in order to accurately calculate the mass of glucose in each standard solution.
 

What is the purpose of finding mass of glucose in standard solutions?

The purpose of finding the mass of glucose in standard solutions is to determine the concentration of glucose in a solution, which is an important factor in many scientific experiments and processes.

How is the mass of glucose in standard solutions determined?

The mass of glucose in standard solutions can be determined through various methods, such as gravimetric analysis, titration, or spectrophotometry. These methods involve measuring the amount of glucose present in a given volume of solution and using mathematical calculations to determine the mass.

Why is it important to use standard solutions when finding the mass of glucose?

Standard solutions are solutions with a known concentration of a specific substance, in this case, glucose. By using standard solutions, scientists can accurately measure the amount of glucose present in a solution and ensure consistency and reliability in their experiments and results.

What factors can affect the accuracy of finding the mass of glucose in standard solutions?

There are several factors that can affect the accuracy of finding the mass of glucose in standard solutions, such as human error, equipment limitations, and environmental factors. It is important to carefully follow experimental procedures and use precise measuring tools to minimize these potential sources of error.

How can the mass of glucose in standard solutions be used in other scientific studies?

The mass of glucose in standard solutions can be used in various scientific studies, such as in biochemistry, medicine, and food science. It can provide valuable information about the concentration of glucose in a substance and its effects on biological processes, health, and nutrition.

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