- #1
uzi
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i need to create a solution of 0.70M Tris HCL buffer of pH 7.9 using 12.1 M of HCL
pKa = 8.3
so far this is what i have:
I need 8.49 g of Tris HCL because:
...(o.7M)*0.1L *(121.4g/mol)
THIS part of the calculation is right so don't worry about it. however i can't get the ratios right.
pH = pKa + log (base/acid)
7.9=8.3 + log (Trisb/Trisa)
7.9-8.3 = log (Tb/Ta)
-04 = log (Tb/Ta)
antilog -0.4 = 0.4
There are 4parts acid to 1 part base therefore:
Tb = 0.4Ta
Total tris = 0.70M
ThereforeL 0.7M =Tb +Ta
0.7 = 0.4Ta + Ta
0.7 = 0.4(4 parts Ta) + 0.4
0.7 = 2Ta
hence:
Ta = 0.7M/2 = 035
Tb = 0.70M - 0.35 = 0.35
BUT this doesn't make sense, because if youplug the ratios into the HH equation you don't get the right pH:
pH = 8.3 + log (0.35/0.35)
pH = 8.3 + 0
pH = 8.3
I need to make it a pH of 7.9 !
and then on top of that since the ratios are wrong I can't successfully calculate the HCL volume. T.T
The only correct ratios that would make sense are .2 and .5, but I did that through trial and error therefore I cannot use it.
pH = 8.3 + log (0.2/0.5)
pH = 8.3 + (-0.397)
pH = 7.9
pKa = 8.3
so far this is what i have:
I need 8.49 g of Tris HCL because:
...(o.7M)*0.1L *(121.4g/mol)
THIS part of the calculation is right so don't worry about it. however i can't get the ratios right.
pH = pKa + log (base/acid)
7.9=8.3 + log (Trisb/Trisa)
7.9-8.3 = log (Tb/Ta)
-04 = log (Tb/Ta)
antilog -0.4 = 0.4
There are 4parts acid to 1 part base therefore:
Tb = 0.4Ta
Total tris = 0.70M
ThereforeL 0.7M =Tb +Ta
0.7 = 0.4Ta + Ta
0.7 = 0.4(4 parts Ta) + 0.4
0.7 = 2Ta
hence:
Ta = 0.7M/2 = 035
Tb = 0.70M - 0.35 = 0.35
BUT this doesn't make sense, because if youplug the ratios into the HH equation you don't get the right pH:
pH = 8.3 + log (0.35/0.35)
pH = 8.3 + 0
pH = 8.3
I need to make it a pH of 7.9 !
and then on top of that since the ratios are wrong I can't successfully calculate the HCL volume. T.T
The only correct ratios that would make sense are .2 and .5, but I did that through trial and error therefore I cannot use it.
pH = 8.3 + log (0.2/0.5)
pH = 8.3 + (-0.397)
pH = 7.9