Simple Plasmid Mapping Problem

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In summary, the given data for the number of base pairs for each restriction enzyme does not allow for a possible plasmid map to be created, unless the number "11" for restriction enzyme B is actually 10.
  • #1
fakecop
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Homework Statement


Use the following data to create a plasmid map.

Number of base pairs for each restriction enzyme
A: 20
B : 11 6 3
A & B: 7 6 3

Homework Equations


The entries under the category "number of base pairs" could repeat themselves as they represent actual data obtained from gel electrophoresis. E.g. 11, 6, 3 could actually be 11, 6, 6, 3

The Attempt at a Solution


I believe this question is impossible due to the number "11" for restriction enzyme B. When both restriction enzymes (A and B) are applied the sequence with length 11 either remains intact or gets chopped up into pieces of length 7, 6, or 3 respectively. But there are no positive integer solutions for the equation 7x+6y+3z=11.
 
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  • #2
Therefore, it is not possible to create a plasmid map with these restrictions.

However, if we assume that the data provided is not completely accurate and 11 is actually 10, then a possible plasmid map could be created as follows:

Plasmid Map:

A: 20 bp
B: 10 bp
A & B: 7 bp - 6 bp - 3 bp

This map assumes that restriction enzyme A cuts the plasmid at 20 base pairs and restriction enzyme B cuts at 10 base pairs. When both enzymes are applied, the resulting fragments are 7, 6, and 3 base pairs in length. This would fit the data provided and create a possible plasmid map. However, without further clarification on the accuracy of the data, this solution is only speculative.
 

1. What is a plasmid?

A plasmid is a small, circular piece of DNA that can replicate independently from the host cell's chromosomal DNA. Plasmids are commonly found in bacteria and are often used in genetic engineering and biotechnology.

2. What is a plasmid mapping problem?

A plasmid mapping problem is a common task in molecular biology where the goal is to determine the relative positions of different restriction enzyme cleavage sites on a plasmid. This information is important for understanding the structure and function of the plasmid.

3. How is a plasmid mapping problem solved?

A plasmid mapping problem is typically solved by using restriction enzyme digestion and gel electrophoresis. The plasmid is cut with different restriction enzymes, and the resulting fragments are separated by size on a gel. The positions of the fragments are then determined by comparing them to a known plasmid map.

4. Why is plasmid mapping important?

Plasmid mapping is important because it provides valuable information about the structure and function of a plasmid. It can help researchers understand how genes are organized on the plasmid and how they are expressed. It is also useful for designing experiments and determining the effects of genetic modifications.

5. What are the applications of plasmid mapping?

Plasmid mapping has many applications in molecular biology and biotechnology. It is commonly used in genetic engineering to determine the structure of a plasmid before making modifications. It is also important for studying gene expression, understanding antibiotic resistance mechanisms, and developing new treatments for genetic diseases.

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