Recent content by Kalirren

Yes, it is a consequence of the reaction mechanism. The point of the "evolutionary limitation" is that evolution works with what it's got. What we -have- is a 5' -> 3' elongation mechanism, not a 3' -> 5' one. So this entire machinery surrounding Okazaki fragments gets invented to deal with...

Yeah, pretty much. The helicase unwinds the DNA, resulting in two single strands of DNA running in opposite directions at the fork. If DNA synthesis can only proceed in one direction, then only one of these can be continuously synthesized, since the other strand must be being unwound in the...

Could you explain why you think your answer is right? More generally, what should happen after each replication (first, second) under each of the three hypotheses? That's only 6 things, you should be able to do that in one post.

Two comments/questions: Why do you believe that the O of Ser195 would be nucleophilic before its proton was partially abstracted by His57? Why do you believe that the substrate would remain in the reaction center if it is no longer involved in the reaction after step c?

Polyethylene teraphthalate has two structural units but only has one repeating unit. Structural units are substructures that are derived directly from the inputs. If you call the repeating unit [-R-], then you can write a polymer as -[-R-]-n. So while polyethylene teraphthalate has the...

This isn't about Punnett squares. Punnett squares are for predicting the genetic makeup of progeny, when given gametic frequency. You are given a recombination frequency and are asked to find gametic frequencies. This is about homologous recombination.

You're already explained what -happened- just fine. What happened to the enzyme in each test tube? Also, this should be in the homework forum.

There are -a lot- of theories regarding the matter, and the murkiness of the issue is complicated somewhat by the uncertainty of estimates regarding the exact dates during which the Earth was entirely covered in ice. We're much more certain about the dates for the radiation of metazoa than we...

I can't think of a reason why any given primer should work only for cDNA. Maybe the reason why you're having trouble with the genomic DNA amplification has to do with other possible annealing sites on the genomic DNA? I had this problem before. If you have upstream/downstream genetic...

The short answer is yes, the chromatin DNA must unwind from its nucleosome-bound state in order to engage in homologous recombination.

It would help if you copied the entire question. I don't understand the ADP addition experiment from what you copied alone.

Hey, I wish I'd thought of doing this when I was trying to write papers. I do have a few suggestions, some requests for clarification, and at least one actual bone to pick. Minor stuff first: Do not capitalize cis-3-Hexenal. Do use consistent formatting in your introduction of supplementary...

So I looked up some papers which had better diagrams, and you're absolutely right, the L pigment -does- have another peak inside the visible. Both the L and the M secondary peaks are right underneath the primary peak of the S, and the S pigment has a secondary peak even further into the shorter...

So I read up some on Wikipedia, and I came up with a partial explanation. Violet and purple are not the same thing. Violet is a "spectral color," meaning that there is monochromatic light of a certain wavelength that looks violet. "Purple," on the other hand, corresponds to no single...

Hm. It seems to me that the "octaves" idea actually wouldn't work, and here's why I think so; The rhodopsin-based pigments work because a cis-trans double-bond isomerization in the rhodopsin center causes a conformational change in the protein, which gets converted into a membrane potential...