Recent content by KKAK

  1. K

    Human Leukocyte Antigen (HLA) typing

    Hi all, I am a little bit confused about the concept behind Human Leukocyte Antigen (HLA). Part of my project right now requires me to do HLA typing from the blood samples with flow cytometry. Here are my questions. 1. The difference between between HLA class I & II compare to MHC Class I &...
  2. K

    Ammonium hydroxide Percent to normality conversion

    Hi all, I was trying to make a 0.3N ammonium hydroxide solution from the stock (Fluka). The label on the stock says it is "10% assay in water", what does it mean? Is it 10% v/v? How should I prepare a 1ml 0.3N ammonium hydroxide? I don't quite understand the solute mass equivalent concept of...
  3. K

    How Do You Calculate Thermodynamic Properties in Different Expansion Processes?

    Hi all , I have 2 questions, I don't know how to derive the equations, please help. Thank you. 1)A reversible adiabatic expansion of 2.5 moles or He from a volume 9L at 300K to a final volume of 28L. Find work(W), heat(Q), internal energy(U) and enthalpy change(H) and final temperature. (He...
  4. K

    Thermal Gravimetric Analysis question.

    Hello, how do we determine the metal weight percent of gold nanopartilces that are coated organic thin films by using thermal gravimetric analysis ?
  5. K

    Relative basicity of two molecules.

    Hi can someone explain to me why is N-Acetylpiperidine is a weaker base than 1-aza-2-2oxobicyclo[2.2.2]octane ? Is it something about the ability to protonate the molecules ? thank you
  6. K

    How can H-NMR graph peaks be used to determine enol-keto ratio?

    Here is an example I found on the internet with the result. I don't know how they get this result. The product I will be getting to analyze is 2-acetylcyclohexanone, I want to understand the concept before I go ahead and do it. thank you file is zipped with a *.jpeg file, no virus.
  7. K

    How can H-NMR graph peaks be used to determine enol-keto ratio?

    Hi can someone help me on how to determine the enol and keto content from a H-NMR graph ? I know we have to use the integration of the peaks to find the ratio, but I just don't quite get it(like which peaks I should use). It would be great if someone can provide a detailed example. Thanks
  8. K

    Organic Chemistry Lab Questions: pKa of p-Chlorophenol

    Thank you chem_tr and altered-gravity. Yeah, after you explained it, I realized that he did mention something similar in his notes, I just didn't make the right connections. Now it makes a lot more sense ! thanks
  9. K

    Organic Chemistry Lab Questions: pKa of p-Chlorophenol

    Isosbestic point is a point on the UV spectrum where all speectrum intersects. By the way , I think you are trying to post a link ? it didn't show up. Thank you.
  10. K

    Organic Chemistry Lab Questions: pKa of p-Chlorophenol

    Hi all I am doing this lab in my organic course right now, and I have a few questions. The phenol I am working on is pKa of p-chlorophenol. I obtained a UV spectrum of phenol in acid, phenol in base, phenol in buffer with higher pH and phenol in buffer with lower pH. 1)In the UV spectrum...
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