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Calculating pH of buffer after adding HCl
Homework Statement If the Tris buffer was exactly pH=9.0, calculate expected pH value after addition of 1 ml of 0.05 HCl. Buffer: 4 ml of 0.01M Tris, pH 9.0 HCl: 1 ml of 0.05M HCl Homework Equations H-H: pH=pKa+log[A-]/[HA] The Attempt at a Solution 9.0=8.21+log[A-]/[HA]...- MissSpice
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- Buffer Hcl Ph
- Replies: 1
- Forum: Biology and Chemistry Homework Help
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Volume of Tris Needed to Prepare Buffer Solution
It's a base. So I would use the 1.0 N HCl to generate the acid, Tris+?- MissSpice
- Post #5
- Forum: Biology and Chemistry Homework Help
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Volume of Tris Needed to Prepare Buffer Solution
I would need .005 mols, (0.1M Tris)x(0.05L)=.005. But then how do I go back to ml from here? And where does the 1.0M Tris base come in, since that is the solution I am diluting to get the 0.1M solution? EDIT: I think I figured it out. Would it be (1.0M Tris)(X L)=(0.1M Tris)(.05L), so I would...- MissSpice
- Post #3
- Forum: Biology and Chemistry Homework Help
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Volume of Tris Needed to Prepare Buffer Solution
Homework Statement Calculate the theoretical volumes of 1.0 M Tris base, 1.0 N HCL or 0.2 NaOH, and water needed to prepare 50 ml of a 0.1 M Tris buffer pH 9.0. pKa of Tris: 8.21 Homework Equations Henderson-Hasselbalch: pH=pKa + log[A-]/[HA]The Attempt at a Solution Honestly, I don't even know...- MissSpice
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- Buffer Prepare Volume
- Replies: 5
- Forum: Biology and Chemistry Homework Help