# Recent content by Thrax

1. ### Slope of the line: Wheat germ extract

Well, your not alone! ( : I was going to post the steps before but i thought it would be to much. But, I'm post them so you can have a look see...Line 16 through 18 get to the heart of what i need to find. Trying to find what volume to multiple by is a bit tricky too. Mixed and diluted the...
2. ### Slope of the line: Wheat germ extract

Hi tiny-tim Thanks for the mu: µ, Let me see if I understand this correctly. if the acid enzyme is 1.022 times the pure enzyme concentration of 1mg/mL, that would give 1.022 x 1mg/mL = 1.022 mg/mL correct? I believe the steeper the slope for the extract indicates that I extracted more...
3. ### Slope of the line: Wheat germ extract

Maybe if i show you what i did it might help. Extraction and Quantification of Acid Phosphatase Enzyme from Wheat Germ 1. Obtain a 15 mL conical centrifugation tube and add 0.5 grams of raw wheat germ to the tube. 2. Add 5 mL of 0.05M Sodium Acetate buffer, pH 4.5. Cap and shake the...
4. ### Slope of the line: Wheat germ extract

The pure anzyme was given at a 1mg/mL concentration. … The question is: How much (ug) of acid phosphatase enzyme was extracted from the wheat germ? I believe the 1.022 is in (mg/mL). Dont I need to multiply this by a volume to get to a mass? Or is this the mass?
5. ### Calculate the molar concentration of substrate

Thanks for taking a look!
6. ### Calculate the molar concentration of substrate

Homework Statement Hello, I'm creating a Michealis/Menten curve and need to find the X values for the graph. Homework Equations These are the instructions given to find the X values: To obtain the X value, plot the molar concentration (molarity) of substrate used in each well...
7. ### Two Lenses

Homework Statement The lenses are separated by 250mm. Where should the object be places so that the final image is formed at infinity? I'm confused.
8. ### Kirchhoff's circuit law

One more thing...This might be a stupid question. But once you've found I1 and I2. If one of those is a negative number...it indicates that the current is flowing in the other direction. So, if I3 = I1 + I2 and let’s say that I1=-1, and I2 = 1would that change the equation to I3= I1-I2 giving...
9. ### Kirchhoff's circuit law

Oh yeah...i see it now. Thanks!!!!!!
10. ### Kirchhoff's circuit law

I3 is 2A, but i dont see the I1-I2 in this situation. Arnt I1 and I2 both converving onto I3? Or Im I looking at it wrong?
11. ### Kirchhoff's circuit law

Sorry, typed that in wrong...I've got -1A for I2, 1A for I1 and 0? for I3.
12. ### Kirchhoff's circuit law

I've got -1A for I1, 1A for I2 and 2A for I3
13. ### Kirchhoff's circuit law

Yes, I believe i do. Let me take a stab at it and see what trouble i can get into...
14. ### Kirchhoff's circuit law

I'm not sure what to do when i hit a battery. I know that you assign a positive value to the battery if the battery output follows the tracing direction. But, for some reason it dosnt always work out. Like you see in this problem....
15. ### Kirchhoff's circuit law

Well, I use whatever gets me there...But, the short line is negative.