Well, your not alone! ( :
I was going to post the steps before but i thought it would be to much. But, I'm post them so you can have a look see...Line 16 through 18 get to the heart of what i need to find. Trying to find what volume to multiple by is a bit tricky too. Mixed and diluted the...
Thanks for the mu: µ,
Let me see if I understand this correctly. if the acid enzyme is 1.022 times the pure enzyme concentration of 1mg/mL, that would give 1.022 x 1mg/mL = 1.022 mg/mL correct? I believe the steeper the slope for the extract indicates that I extracted more...
Maybe if i show you what i did it might help.
Extraction and Quantification of Acid Phosphatase Enzyme from Wheat
1. Obtain a 15 mL conical centrifugation tube and add 0.5 grams of raw
wheat germ to the tube.
2. Add 5 mL of 0.05M Sodium Acetate buffer, pH 4.5. Cap and shake the...
The pure anzyme was given at a 1mg/mL concentration.
The question is: How much (ug) of acid phosphatase enzyme was extracted from the wheat germ?
I believe the 1.022 is in (mg/mL). Dont I need to multiply this by a volume to get to a mass?
Or is this the mass?
I'm creating a Michealis/Menten curve and need to find the X values for the graph.
These are the instructions given to find the X values:
To obtain the X value, plot the molar concentration (molarity) of substrate used in each well...
One more thing...This might be a stupid question. But once you've found I1 and I2. If one of those is a negative number...it indicates that the current is flowing in the other direction. So, if I3 = I1 + I2 and let’s say that I1=-1, and I2 = 1would that change the equation to
I3= I1-I2 giving...
I'm not sure what to do when i hit a battery. I know that you assign a positive value to the battery if the battery output follows the tracing direction. But, for some reason it dosnt always work out. Like you see in this problem....