SUMMARY
The Bradford method requires two blanks of 0.15M NaCl to ensure accurate photometric measurements. One blank is used to zero the photometer with water, while the second blank is prepared with Coomassie Brilliant Blue to measure absorption. This approach allows for the subtraction of the blank's absorption from the standards when plotting the standard curve. Additionally, serial dilutions of the unknown sample are necessary to ensure that the concentration falls within the detectable range of the assay.
PREREQUISITES
- Understanding of the Bradford assay methodology
- Familiarity with photometric measurement techniques
- Knowledge of Coomassie Brilliant Blue properties
- Experience with serial dilution techniques
NEXT STEPS
- Research the principles of the Bradford assay and its applications
- Learn about photometer calibration and zeroing techniques
- Study the preparation and use of Coomassie Brilliant Blue in assays
- Explore serial dilution methods and their importance in quantitative analysis
USEFUL FOR
Laboratory technicians, biochemists, and students conducting protein assays or working with spectrophotometric analysis.