A question about biological molecule's fluorescence

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Discussion Overview

The discussion revolves around the fluorescence of biological molecules, specifically focusing on their quantum fields and examples of molecules that exhibit strong fluorescence. Participants explore various aspects of fluorescence, including specific molecules and their applications in research.

Discussion Character

  • Exploratory
  • Technical explanation
  • Conceptual clarification
  • Homework-related

Main Points Raised

  • Some participants inquire about the quantum field of biological molecules and seek examples of those that emit strong fluorescence, such as beta-carotene.
  • Green fluorescent protein (GFP) from Aequorea victoria is mentioned as a common example used in research.
  • There is a question about the existence of other molecules that can emit fluorescence in the visible band.
  • One participant explains the fluorescence process, noting that different molecules have unique excitation and emission characteristics, and lists other commonly used fluorophores like phycoerythrin and fluorescein isothiocyanate (FITC).
  • Participants are directed to look for articles in scientific journals that report on the absorption and emission spectra of these compounds.
  • A distinction is made between fluorescence and chemiluminescence, with examples provided for the latter, such as fireflies.

Areas of Agreement / Disagreement

The discussion includes multiple viewpoints and remains unresolved regarding the specific quantum fields of biological molecules and the breadth of examples of fluorescent molecules.

Contextual Notes

Some assumptions about the familiarity with fluorescence and the specific applications in experiments are present, but not explicitly stated. There is also a reliance on older literature for certain compounds like beta-carotene.

Who May Find This Useful

Researchers and students interested in the fluorescence properties of biological molecules and their applications in scientific research may find this discussion relevant.

breezels
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many biological molecule can emit fluorescence.
but is the quantum field of these molecules large?
can u give me some examples that the molecule emits strong fluorescence,
such as beta-carotene? for this field ,i know little.
thank u
 
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An example is green fluorescent protein from Aequorea victoria, used a lot for research purposes.
 
are there some molecules that can emit fluorescence in the visible band?
 
GFP fluoresces in the lower green portion of the visible spectrum, why exactly do you need to know? p.s. Aequorea victoria is a yelly fish.
 
For something like GFP, you can find articles in the types of journals that publish new techniques that report the absorption and emission spectra for those compounds. Similar information should also be available for something like beta-carotene, but it's likely to be found in much older literature.
 
Fluorescence is essentially a reaction to existing light energy, the molecule is excited by visible light or other wavelengths, it resides inthe excited state for a time and then emits some of that energy back out at longer wavelengths. Different molecules excite and emit with different characteristics. The green fluorescent protein mentioned in previous posts is only one of many biologically and synthetically produced compounds that are used in science to monitor numerous cellular and subcellular endpoints such as presence of surface markers, reporter gene activity, pH, calcium concentrations, etc. Some other commonly used fluorophores are phycoerythrin, fluorescein isothiocynate (FITC), PerCP and others. Check the information of a site from a company called http://probes.invitrogen.com/handbook/sections/0001.html"for a catalog of these chemicals used in science and their applications/references
This process differs from chemiluminescence that generates it's own light via chemical reactions. Fireflies and deep sea organisms are good examples of applications of this process.
 
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because a experiment need that
 

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