Bacterial colonies in DNA cloning?

  • #1
sameeralord
662
3
If there is some identical bacteria in petri dish and you insert plasmids containing different DNA fragemnts. When bacteria that takes it up grows, do they grow in separate colonies. I mean does all bacteria that took plasmid A grow into one colony, and all bacteria that got plasmid B into one colony. But at the beginning all the bacteria was identical so why do they grow into separate colonies. Basically in other words I don't understand how bacterial colonies work. Thanks :smile:
 

Answers and Replies

  • #2
Ygggdrasil
Science Advisor
Insights Author
Gold Member
2021 Award
3,522
4,179
The way you isolate bacterial colonies is by diluting the bacteria down to a low concentration and then spreading them out on a petri dish. This way, individual bacterial cells are spread out throughout the petri dish and relatively far away from their neighbors. As the bacteria divide, the progeny of each individual bacteria are spatially separated from each other and form colonies on the plate.
 
  • #3
sameeralord
662
3
The way you isolate bacterial colonies is by diluting the bacteria down to a low concentration and then spreading them out on a petri dish. This way, individual bacterial cells are spread out throughout the petri dish and relatively far away from their neighbors. As the bacteria divide, the progeny of each individual bacteria are spatially separated from each other and form colonies on the plate.

Thanks :smile: Does this mean, let's say some bacteria took plasmid A and some took plasmid B. Then dilute as you said. Then can there be like 2 or more colonies contaning plasmid A or B.
 
  • #4
Ygggdrasil
Science Advisor
Insights Author
Gold Member
2021 Award
3,522
4,179
Yes. The number of colonies with the plasmid will reflect the number of bacteria that took up the plasmid.
 

Suggested for: Bacterial colonies in DNA cloning?

  • Last Post
Replies
12
Views
763
Replies
1
Views
365
  • Last Post
Replies
2
Views
443
  • Last Post
Replies
2
Views
647
  • Last Post
Replies
3
Views
473
Replies
4
Views
526
  • Last Post
Replies
1
Views
2K
  • Last Post
Replies
2
Views
646
  • Last Post
Replies
4
Views
2K
Replies
1
Views
598
Top