Beer Lambert Law: Calculate Molar Extinction Coefficient at 405nm

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SUMMARY

The discussion focuses on calculating the molar extinction coefficient of the p-nitrophenolate ion at 405nm using the Beer-Lambert Law. The absorbance of a diluted solution (74μM diluted 1:2) was measured at 0.650. The equation A = εcl was utilized, where A is absorbance, ε is the molar extinction coefficient, c is concentration, and l is the path length. It was emphasized that the units must be included to correctly determine the units of ε, which are typically L cm-1 mol-1.

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Homework Statement


The p-nitrophenolate ion absorbs lights at 405nm. A solution that is 74um in the p-nitrophenolate ion was diluted 1 in 2 with water.Absorbance of resulting solution at 405nm was .650. Calculate molar extinction coefficient of the p-nitrophenolate ion at 405nm. Your meant to assume water has no absorbance at 405 nm.

How do you use the beer lambert law to predict the absorbance at 405nm of a solution that is 11.0mM in the p-nitrophenolate ion, how do you show absorbance has no units


Homework Equations


A=ecl


The Attempt at a Solution



.650 = e x 1 x (74/2)??
dont think I am right at all...
 
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That's the correct way to setup the equation, but you should include units so that you can get the units of ε correct (ε is usually reported in units of L cm-1 mol-1).
 

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