I need to calculate molar extinction coefficient for Albumin at 280nm.
But since L=constant, I can just say ε=A/c
The Attempt at a Solution
The given molecular mass of albumin is 66,5kDa, which equals 66500g/mol. I do understand that I can find c (concentration) via the molecular mass. What I don't understand is how can I find ε if I still have another unknown, which in this case happens to be A.
Have I missed something really simple and overthinking this for myself? How can I find Absorbance?