Dismiss Notice
Join Physics Forums Today!
The friendliest, high quality science and math community on the planet! Everyone who loves science is here!

Dear allI work with a spectrophotometer

  1. Jul 23, 2011 #1
    dear all
    I work with a spectrophotometer "Carry 17D". below 300nm wavelenght the transition spectrom have lots of flactuation. how to solve the problem and how to provide a clear spectrom?
  2. jcsd
  3. Jul 29, 2011 #2

    Claude Bile

    User Avatar
    Science Advisor

    Re: spectrophotometer

    Often spectrometers will compute a baseline and then normalise spectra to that baseline. If the signal to noise ratio drops at shorter wavelengths, then normalised spectra will become progressively noisier at the shorter wavelengths.

    To resolve this, you need either a brighter source, a better detector, or optics/gratings that have lower losses below 300 nm.

    Measuring lots of spectra and averaging them can also reduce the noise, but this approach is time-consuming.

Share this great discussion with others via Reddit, Google+, Twitter, or Facebook